Free ASCP ASCP-MLT Practice Exam with Questions & Answers | Set: 3

Waived tests are those considered to have an insignificant risk of erroneous results. Which of the following is NOT an example of a waived test?

Gram positive organisms resist decolorization by ethyl alcohol. The large crystal violet-iodine complex is not able to penetrate the peptidoglycan layer, and is trapped within the cell in gram-positive organisms. Conversely, the outer membrane of gram-negative organisms is degraded and the thinner peptidoglycan layer of gram-negative cells is unable to retain the crystal violet-iodine complex and the color is lost.

What is the purpose of using ethyl alcohol or acetone in the gram stain procedure:

Phase of reactivity is primarily at immediate spin (4+) and reactions get weaker at AHG (w+). There is no specific pattern of reactivity and the auto control is negative which rules out an autoantibody. This is a strong cold antibody which is still slightly present after incubation and washing.

Activation and binding of the antibody takes place at room temperature or colder. Eliminating this phase will prevent the antibody from binding. Cold antibodies usually are more of a nuisance to blood bankers and are not clinically significant.

When performing an antibody screen, both the screen cells are 4+ at immediate spin and W+ at AHG. The antibody panel shows 4+ reactions at immediate spin and W+ reactions at AHG and there is no specific match to the reaction pattern. The auto control is negative. What would be a logical next step?

hs-CRP is a more sensitive version of the C-reactive protein (CRP) test, a test that has been used for many years to assess inflammation in settings such as lupus, transplantation, infection, etc.

Which of the following cardiovascular risk markers is a more sensitive version of a test that is used to assess inflammation?

The difference between glass and plastic red stopper tubes is that the:

Question options:

CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep). The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.

Microbiology

Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of bacitracin was noted. The other test necessary to identify this organism is a positive test by:

Medical ethics

hs-CRP is a recent marker of chronic inflammation. New measurements of CRP in lower levels (hs-CRP) are now measured to monitor risk of cardiovascular disease.

Select the statement that best describes hs-CRP?

In DIC, or disseminated intravascular coagulation, the prothrombin time is increased due to the consumption of the coagulation factors due to the tiny clots forming throughout the vasculature. This is also the reason that the fibrinogen levels and platelet levels are decreased. Finally FDP, or fibrin degredation products, are increased due to the formation and subsequent dissolving of many tiny clots in the vasculature. The FDPs are the pieces of fibrin that are left after the fibrinolytic processes take place.

Which of the following laboratory results would be seen in a patient with acute Disseminated Intravascular Coagulation (DIC)?

IgE levels are often increased in patients with allergic disease. IgE binds to the membranes of mast cells and basophils, and if specific antigen is present to react with the IgE molecule, degranulation of these cells occurs, releasing histamines, and other substances into the blood or tissues.

Which of the following immunoglobulin classes is chiefly responsible for the degranulation of mast cells and basophils:

Specimens collected from patients in contact isolation should be:

The mandatory waiting period between blood donations is 56 days, or eight weeks.

The accepted interval between blood donations is:

Chain-of-Custody procedures must be followed for

Hepatitis B surface antigen, or HBs, is a protein that is present on the surface of the virus. The protein will be present via laboratory testing in the blood with acute and chronic HBV infections

Immunology

What sero-marker is the first marker to become positive in Hepatitis B and is associated with the infective stage?

The approximate volume of CSF in an adult is 90-150 mL.

What is the approximate volume of spinal fluid in an adult?